Phenotypical characterization of the peripheral blood T cells in patients with celiac disease: does it differentiate suspicious celiac disease cases?

The present study aimed to study the immunological changes seen in the intestinal epithelium of the celiac patients could also be detected in the peripheral blood lymphocyte populations. Celiac disease [CD] is a small bowel enteropathy caused by permanent wheat gluten intolerance. One of the earliest signs of CD is an increase in the numbers of the intestinal intraepithelial lymphocytes [iIEL]. In this case-control study, totally 13 untreated subjects with acceptable criteria for CD without any complication and 16 healthy subjects without any positive criteria for CD were selected. Peripheral blood T cells were analyzed by two-color flow cytometry in both groups. The mean age of patients was 33.6 +/- 3.4 years and two patients had Marsh IIIB, five patients had Marsh IIIA and six patients had Marsh II histology class. The mean percentages of the gamma delta]TCR[+] T cells in the patients were significantly higher than the controls [p=0.015]. However, the mean percentages of the [alpha beta]TCR[+] T cells were significantly lower in the untreated patients than the controls [p=0.025]. There were no significant difference between the mean percentages of lymphocytes expressing the CD3, CD4 and CD8 molecules in the patients and the controls. The change in the percentages of the peripheral blood T cells expressing the [gamma delta]TCR and [alpha beta]TCR in the celiac patients could be used in conjunction with the other serological markers to identify new CD cases

[Evaluation of the mean percentages of the peripheral blood gamma delta t lymphocytes in the healthy subjects and celiac patients in Isfahan]

An elevation in the mean percentages of the gamma delta T lymphocytes per total T cells in the epithelium of the small intestine has a diagnostic value for celiac disease. This study aimed to measure the percentages of the peripheral blood gamma delta T lymphocytes in the adults' celiac disease and healthy controls.In this case-control study the absolute numbers of the peripheral blood lymphocytes obtained from the controls [n = 21] and celiac patients [n=15] were studied, Using a cell counter. The proportions of the gamma-delta T lymphocytes were evaluated by the flow cytometer method. The results showed that there was not significant I difference in the percentages of the gamma-delta T lymphocytes between celiac patients and healthy individuals. [p value= 0.84] Collectively, the data show that the percentages of the peripheral blood gamma delta T lymphocytes could not be helpful for celiac disease diagnosis

effect of aspirin on serum vascular endothelial growth factor and nitric oxide concentration in high-cholesterol fed rabbits

Hypercholesterolemia is one of the major risk factors for atherosclerosis which is characterized by endothelial dysfunction. This study was designed to investigate the effect of aspirin on serum vascular endothelial growth factor [VEGF] and nitric oxide [NO] concentrations in hypercholesterolemic animals. Sixteen male rabbits were randomly divided into two groups, aspirin-treated and control. Aspirin [10 mg/kg/day] was administered orally using feeding tube. All animals were fed with high-cholesterol diet [1%] during the experiment. After five weeks, blood pressure, serum lipid and lipoprotein profiles, serum VEGF and NO concentrations were measured. Aspirin did not change blood pressure. Aspirin significantly decreased serum LDL [1276 +/- 72.1 vs. 1505 +/- 68.03 mg/dl] and triglyceride [477.5 +/- 8.3 vs. 649.1 +/- 15.2 mg/dl] [P<0.05]. High-cholesterol diet significantly decreased serum VEGF level in both groups [control: 24.59 +/- 0.42 vs. 38.09 +/- 2.49 pg/ml; aspirin: 24.72 +/- 0.84 vs. 42.29 +/- 2.03 pg/ml] [P<0.05] and aspirin did not change serum VEGF level in hypercholesterolemic animals [P>0.05]. Serum NO concentration was also significantly decreased after five weeks of high-cholesterol diet [control: 5.87 +/- 0.33 vs. 8.67 +/- 0.68 ?mol/lit; aspirin: 5.66 +/- 0.33 vs. 8.58 +/- 0.60 ?mol/lit] [P<0.05]. Aspirin did not change serum NO level [P>0.05]. We conclude that under the conditions of this study, aspirin cannot change serum VEGF and NO concentrations in high-cholesterol fed animals